We are looking for a reliable Production Assistant to join our team. They will undertake production assistant duties and providing adequate support when needed. The ideal candidate will be competent in prioritizing tasks and should be self-motivated and trustworthy.

Work, Duties and Responsibilities include, but are not limited to, the following:

• Understand and follow basic verbal and written instructions.
• Effectively communicate information and respond to questions from various levels within the organization
• Maintain the production pace of other team members
• Move product and raw materials to and from inventory using a forklift and skid steer. Must be certified or become certified in the operation of a forklift and skid steer.
• Report defective materials or questionable conditions on products, raw materials, and issues affecting the smooth operation of the plant to your supervisor.
• Maintain the work area and equipment in a clean and orderly condition and follow prescribed safety regulations
• May be required to stand for long periods of time and work overtime hours if /when required by your supervisor. Also, you would be responsible for cleaning the blender after each production circle.
• May involve some repetitive motions and MUST adhere to the safety protocols in place
• Must wear proper PPE as required by law and management.
• Must be able to lift up to 25-50 pounds.
• May also engage in frequent bending, stopping, squatting, pushing and pulling of parts and part containers.
• Handling chemicals in a safe manner, utilize and understand the Material Safety Data Sheets MSDS to identify and understand chemicals training provided (WHIMS).
• Perform basic visual inspections on products
• Utilize power tools and various equipment as needed to perform duties

Job Type: Full-time
Email resumes: hr@customagintel.com

We are looking for a reliable Office Administrator to join our team. They will undertake administrative duties and providing adequate support when needed. The ideal candidate will be competent in prioritizing and working with little supervision. They will be self-motivated and trustworthy.

Duties and Responsibilities

• Manage calls and correspondence (emails, letters, packages)
• Accommodate visitors, clients, and job candidates
• Coordinate conference room bookings, appointments, and training seminars
• Manage all matters pertaining to reception/office appearance and utilities
• Cooperate with Operations Manager on local facility
• Assist with administrative tasks and provide research and administrative support to all departments and individuals where necessary
• Preparing memos, reports, spreadsheets, etc.
• Accounts payable/receivable
• Managing office budgets
• Using a range of software products – Microsoft Word, Excel and QuickBooks.
• Implementing and maintaining office procedures
• Data entry
• Filing

Key Skills

• Reliability and discretion
• Adaptability
• Organization and time management
• Problem solver
• Leadership
• Attention to detail

Requirements

• 2 years’ experience in a similar role
• QuickBooks experience would be an asset
• Outstanding communication
• Valid Driver’s License and Clean Drivers Abstract
• Mandatory Criminal Record check is required prior to hiring
  

Job Type: Full-time
Email resumes: hr@customagintel.com

What is Pseudomonas fluorescens?

Pseudomonas spp. are bacterial species ubiquitous in agricultural soils, well adapted to growing in the rhizosphere and well suited as biocontrol and growth-promoting agents (Weller et al., 2007). Pseudomonas spp. rapidly utilize root and seed exudates, multiply in rhizosphere and spermoshphere environment and in the interior of the plants, produce a wide spectrum of bioactive metabolites (such as antibiotics, siderophores, volatiles, and growth-promoting substances); adapt to environmental stresses and compete aggressively with pathogens (Weller et al., 2007) (Panpatte et al., 2016). 

How can Pseudomonas spp. help my crops?

Relief from environmental stresses

Colonies of Pseudomonas spp. are aggressive to persist throughout the growing season, limit the amount of minerals and nutrient availability to pathogens for growth, pre-empt and compete with pathogens for the favored sites on the root, and improve plant growth by suppressing major (root or vascular diseases) and minor (parasites or saprophytes) plant pathogens (Weller et al., 2007). Pseudomonas spp. solubilize phosphorous in soil, release chelating agent called siderophores for iron absorption, nitrogen fixing ability, increase in elements availability to plants to enhance plant growth (Shivasakthi et al., 2014) (Panpatte et al., 2016). 

Plants immune system response

Pseudomonas spp. demonstrate dynamics of root colonization depicting high microbial diversity, density, synchronized metabolic activity, formidable biological buffering capacity that not only protect from foreign pathogens but also from the introduced pathogens (Weller et al., 2007). During the plant growth application, Pseudomonas spp. produce a potent antibiotic resulting in control and suppression of bacterial disease fire blight even in areas with streptomycin-resistant populations of the pathogen and provide moderate to excellent efficacy (Couillerot et al., 2008) as represented in figure 1. Pseudomonas spp. produce antimicrobial and metabolites, such as 2,4-diacetylphloroglucinol (DAPG), phenazines, hydrogen cyanide and surfactants, which have received extensive attention and efficacy rate to inhibit various phytopathogens. Pseudomonas spp. also produce extracellular lytic enzymes for protection of plant species and induce an ISR response in plants, and to make plants efficient in fighting back against pathogens (Couillerot et al., 2008).

Plant disease management

The secretion of anti-fungal metabolites by Pseudomonas spp. effectively inhibited mycelial growth by 62-85% and at increased metabolite concentration of 5% completely inhibited the growth of fungal pathogens such as Rhizoctonia bataticola, Fusaricum oxysporum, Xanthomonas oryzae, Rhizoctonia solani (bacterial leaf blight (BB) and sheath blight (ShB) pathogens of rice) (Shivasakthi et al., 2014). Pseudomonas spp. shows high efficacy in controlling the pathogen infections in immature pear fruit, flowers, and whole plants (Pujol et al., 2006). Pseudomonas spp. are also applied in the packinghouses to prevent postharvest fungal disease during storage of citrus, pome, stone fruits, and potatoes (Stockwell et al., 2007). 

Should I inoculate my crops with Pseudomonas spp.?

Increased crop yield

The application of Pseudomonas spp.  as seed inoculants on crop plants rapidly colonize plant roots, promote growth, enhance crop yield by direct and indirect mechanisms, increase in fresh and dry plant masses resulting in increased yield by 44% (Shivasakthi et al., 2014) (Panpatte et al., 2016). Field experiments on chickpea treated with Bacillus and Pseudomonas spp. delivered maximum significant increase in nodule number, dry weight of nodule, root and shoot and due to additional accumulation of phosphorus, iron, and plant hormones like indole-3-acetic acid (IAA) (Verma et al., 2010).

In presence of Pseudomonas spp. the canola plants delivered increased uptake of phosphorous, improved root (61-65%) and shoot (33-47%) elongation, and promoted plant growth under the gnotobiotic conditions (Lifshitz et al., 1987). In case of soyabean, the Pseudomonas spp. treatment resulted in increased shoot (14-26%) and yield (9-36%) (Schmidt et al., 2015), in alfafa increased the alginate biosynthesis by 3-fold and decreased the disease infection frequency by 50% (Silo-Suh et al., 2002). In chickpeas increased the biochemical properties of the crop under the soil contaminated with chromium (Oves et al., 2013) and reduction in plant mortality by (63.3-100%) (Negi et al., 2008). Application of Pseudomonas spp. on lentils cultivated in soil depleted with nutrients, resulted in 85% of nodule formation, higher nodule occupancy and delivered increased nitrogen fixation (Sepúlveda-Caamaño et al., 2018) and seed treatment with foliar applications improved yield in lentils (Erdemci et al., 2020).

Will my crop management practice impact Pseudomonas spp. growth?

Crop management practise such as crop rotations, tillage, seed variety, seeding rate, sanitation, summerfallow, trap strips, soil fertility, intercrops, biological control, pest, and insect management etc. (Crop Management, 2020) have little impact on the effectiveness of Pseudomonas spp. season-to season. The Pseudomonas spp. communities are affected long term by plant species and genotypes and through short-term effects during the lifespan of single plant (Schmidt et al., 2015). The current findings on the Pseudomonas spp. are gaining broader incorporation into sustainable agriculture strategies for the crop management against soil pathogens and postharvest disease (Weller et al., 2007).

Can I use Pseudomonas spp. with other inoculants at the same time?

Pseudomonas spp. can be used simultaneously along with biocontrol inoculants, which posses the property of forming plant growth promoting rhizobacteria (PGPRs) such as Bacillus, Pseudomonas, and Trichoderma (Panpatte et al., 2016). The Pseudomonas spp. plays a major role in PGPRs to deliver benefits across plant growth promotion and suppression of diseases (Negi et al., 2008) and work simultaneously with other species (Bacillus, and Trichoderma) to increase the availability of phosphorus and nitrogen fixation in soil to host plant (Schmidt et al., 2015) (Shivasakthi et al., 2014).

References:

1. Pujol, M., Badosa, E., Manceau, C., & Montesinos, E. (2006). Assessment of the environmental fate of the biological control agent of fire blight, Pseudomonas fluorescens EPS62e, on apple by culture and real-time PCR methods. Applied and Environmental Microbiology, 72(4), 2421-2427.
2. Weller, D. M. (2007). Pseudomonas biocontrol agents of soilborne pathogens: looking back over 30 years. Phytopathology, 97(2), 250-256.
3. Stockwell, V. O., & Stack, J. P. (2007). Using Pseudomonas spp. for integrated biological control. Phytopathology, 97(2), 244-249.
4. Couillerot, O., Prigent‐Combaret, C., Caballero‐Mellado, J., & Moënne‐Loccoz, Y. (2009). Pseudomonas fluorescens and closely related fluorescent pseudomonads as biocontrol agents of soil‐borne phytopathogens. Letters in applied microbiology, 48(5), 505-512.
5. Sivasakthi, S., Usharani, G., & Saranraj, P. (2014). Biocontrol potentiality of plant growth promoting bacteria (PGPR)-Pseudomonas fluorescens and Bacillus subtilis: a review. African journal of agricultural research, 9(16), 1265-1277.
6. Panpatte, D. G., Jhala, Y. K., Shelat, H. N., & Vyas, R. V. (2016). Pseudomonas fluorescens: a promising biocontrol agent and PGPR for sustainable agriculture. In Microbial inoculants in sustainable agricultural productivity (pp. 257-270). Springer, New Delhi.
7. Verma, J. P., Yadav, J., & Tiwari, K. N. (2010). Application of Rhizobium sp. BHURC01 and plant growth promoting rhizobactria on nodulation, plant biomass and yields of chickpea (Cicer arietinum L.). International Journal of Agricultural Research, 5(3), 148-156.
8. Lifshitz, R., Kloepper, J. W., Kozlowski, M., Simonson, C., Carlson, J., Tipping, E. M., & Zaleska, I. (1987). Growth promotion of canola (rapeseed) seedlings by a strain of Pseudomonas putida under gnotobiotic conditions. Canadian Journal of Microbiology, 33(5), 390-395.
9. Sepúlveda-Caamaño, M., Gerding, M., Vargas, M., Moya-Elizondo, E., Oyarzúa, P., & Campos, J. (2018). Lentil (Lens culinaris L.) growth promoting rhizobacteria and their effect on nodulation in co-inoculation with rhizobia. Archives of Agronomy and Soil Science, 64(2), 244-256.
10. Erdemci, İ. (2020). Effect of Pseudomonas Fluorescent Rhizobacteria on Growth and Seed Quality in Lentil (Lens Culinaris Medik.). Communications in Soil Science and Plant Analysis, 51(14), 1852-1858.
11. Schmidt, J., Messmer, M., & Wilbois, K. P. (2015). Beneficial microorganisms for soybean (Glycine max (L.) Merr), with a focus on low root-zone temperatures. Plant and soil, 397(1-2), 411-445.
12. Silo-Suh, L., Suh, S. J., Sokol, P. A., & Ohman, D. E. (2002). A simple alfalfa seedling infection model for Pseudomonas aeruginosa strains associated with cystic fibrosis shows AlgT (sigma-22) and RhlR contribute to pathogenesis. Proceedings of the National Academy of Sciences, 99(24), 15699-15704.
13. Oves, M., Khan, M. S., & Zaidi, A. (2013). Chromium reducing and plant growth promoting novel strain Pseudomonas aeruginosa OSG41 enhance chickpea growth in chromium amended soils. European journal of soil biology, 56, 72-83.
14. Negi, Y. K., Garg, S. K., & Kumar, J. (2008). Plant growth promoting and biocontrol activities of cold-tolerant Pseudomonas fluorescens isolates against root rot in pea. Indian Phytopathology, 61(4), 461.
15. Crop Management, (2020) https://www.saskatchewan.ca/business/agriculture-natural-resources-and-industry/agribusiness-farmers-and-ranchers/crops-and-irrigation/organic-crops/organic-crop-management-insect-management

About Author: VINAYAK PACHAPUR, PhD